Urine is a biofluid produced by the kidneys to eliminate waste products such us urea and metabolites from the body by the urinary tract. Urine also contains some epithelial and blood cells, bacteria, viruses and importantly also extracellular vesicles (among them exosomes).
Exosomes isolate from urine mirror molecular processes, both physiological and pathological conditions, that happen in kidney, urothelial and prostate tissue.
Remarkably, urine can be easily and frequently collected in large quantities in a non-invasive manner.
Exosome isolated from urine represent a good surrogate for the identification of informative biomarkers for renal-related diseases.
Easy and quick isolation of exosomes from urine samples with a precise method.
Additional Information
ExoGAG should be stored in darkness. And, prior to use, ExoGAG must be vortex.
Recommended Usage
We recommend to centrifuge the urine at 2.000g for 5 minutes and 4 °C to remove cells and cellular debris. And if additional debris remains detectable, supernatant should be centrifuged again for other 10 minutes. If urine is stored at -80 °C, it must be thawed and tempered on ice before centrifugation. Urine can be stored at 4 °C for 24h.
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