Plasma is the liquid component of the blood. It is obtained after removing its cellular fraction (erythrocytes, leukocytes and thrombocytes). Although, it keeps the coagulation cascade factors.
Plasma is a source of proteins, cell-free nucleic acids, extracellular vesicles (among them exosomes) and metabolites. Indeed, it can host exosomes from any cellular type of the body in both physiological and pathological conditions. Given this, plasma is one of the major sources of exosomes for clinical applications, since liquid biopsies represent a low-invasive procedure.
Exosome isolate from plasma represent a good surrogate for the identification of informative biomarkers for disease diagnosis, prognosis and longitudinal monitoring.
Easy and simple method to obtain exosomes from human plasma with reliable results.
Additional Information
ExoGAG should be stored in darkness. And, prior to use, ExoGAG must be vortex.
Recommended Usage
We recommend to centrifuge the plasma at 2.000g for 5 minutes and 4 °C to remove cells and cellular debris. And if additional debris remains detectable, supernatant should be centrifuged again for other 10 minutes. If plasma is stored at -80 °C, it must be thawed and tempered on ice before centrifugation. Plasma can be stored at 4 °C for 24h.
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